LiTaq™ PCR系列

LiTaq™ PCR系列

LiTaq™ DNA 聚合酶是一种热稳定性 DNA 聚合酶,具有 5'→3' 聚合酶活性和 5'→3' 核酸外切酶活性,但没有 3'→5' 核酸外切酶活性。 LiTaq™ DNA 聚合酶是从过表达栖热菌 DNA 聚合酶基因的大肠杆菌 (E.coli) 菌株中纯化得到的。 在此酶中未检测到核酸内切酶、核酸外切酶或细菌 DNA。

目录号保真度应用
M0024常规PCR
M0025高产量PCR
M002124×热启动PCR,qPCR
M003196×高保真PCR

成功案例

Samples: Human genomic DNA & cDNA, Mouse genomic DNA, Wheat genomic DNA, Rice genomic DNA, Coli colony

LifeSct: LiTaq™ PCR mix, LiTaq™ DNA polymerase, 2× LiTaq™ plus PCR mix, 2× LiTaq™ Eco PCR mix

Competitors: Brand C, Brand N, Brand Q, Brand T, Brand G, Brand F

PCR reaction setup for amplification:
• 2× Mix: 12.5 µl
• F1: 1 µl
• R1 : 1 µl
• Template* : 0.5 µl
• ddH2O: 0.2 
µl
* gDNA: 50 ng; λ DNA: 5 ng; Plasmid: 2 ng;  Colony: 1 µl

PCR cycling for amplification:
1. 95°C: 3 min
2. 35 x
    1. Denaturation: 15 s at 95°C
    2. Annealing: 20 s at 60°C
    3. Extension: 1 min/kb at 72°C
3. Final extension: 5 min at 72°C


从以上结果可以看出,对于不同长度和类型模板的PCR扩增,我公司的四种PCR混合物均表现出色。 对于通用PCR,我们的产品具有最广泛的适用性、最好的成功率、最高的特异性和最大的产量。 在最困难的情况下,当所有其他 PCR 混合物都失败时,我们的至少一种产品仍然有效。

产品名称目录号价格
2× LiTaq™ PCR Master Mix (+Dye) (5×1 ml)M0024¥230
2× LiTaq™ Plus PCR Master Mix (+Dye) (5×1 ml)M0025¥310
2× LiTaq™ HotStart PCR Master Mix (5×1 ml)M0021¥680
2× LiTaq™ SuFi PCR Master Mix (1 ml)M0031¥480

引用文献

1. Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD. 
发表期刊:Nature Communications        产品服务:2× LiTaq™ PCR Master Mix (+Dye)

2. Distinct Structures and Dynamics ofChromatosomes with Different Human LinkerHistone Isoforms.
发表期刊:Molecular Cell        产品服务:2× LiTaq™ PCR Master Mix (+Dye)

2. In Vitro Analysis of Rationally Designed Multimerization Sequences.
发表期刊:ProQuest        产品服务:LiTaq™ Plus PCR Master Mix (+Dye)

客户评价

"Our lab started to use the high-quality services from LifeSct, including the gene synthesis and the gBlocks gene fragments since 2017. We are always happy with the great quality of the services, the reasonable price, the prompt shipment and the good communication from LifeSct. More impressively, your LiTaq™ PCR Master Mix is robust and cost-effective. Thanks for continuing to produce great products and provide excellent services!"

------ NIH/NICHD, Dr. Chune Cao, Lab manager

"I'm citing 2× LiTaq™ PCR Master Mix (LifeSct LLC) in my manuscript for a protein engineering paper. It is extremely effective, and I really appreciated the sample. I'll send you a copy as soon as it's published.BTW, LiScript™ Fast RT Master Mix works great, actually I wanted to mention how fast and easy it was to use. The addition of RNAse inhibitor and the 5x concentration are perfect for fast experiments."

------ San Diego State University, Dr. Brent White, Postdoc

"I have been using LiTaq™ PCR Master Mix and LiQuant™ Universal Green qPCR master mix in my experiments for two years. The PCR mix can impressively amplify the difficult sequences in my hands. Amazingly, it rapidly amplifies the templates at ~ 2kb/min. And the qPCR mix has the higher sensitivity compared with the similar product from other company. I highly recommend these products!!!"

------ UPENN, Dr. Jinping Liu, Research Associate

"Our Lab had a chance to try the LiTaq™ PCR Master Mix and LiClone™ One Step Cloning Kit which perform very well compared with similar products we used before. We will order them later. Thank you!"

------ NDSU, Dr. Ang Guo, Assistant Professor

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