Violet Cell Proliferation Kit (200 rxns)

Violet Cell Proliferation Kit (200 rxns)

目录号 C0015
库存状态 有库存
¥1,800.00
-+
产品优势:

• Superior performance: Bright, single-peak staining enables visualization of multiple generations

• Long-term signal stability: Well-retained in cells for several days post stain.

• Easy multiplexing with other fluorophores

• Simple, robust staining protocol

描述:

Violet Cell Proliferation Kit provides convenient single-use vials for cell labeling. The Cell-ID™ Violet reagent easily diffuses into cells and bind covalently to intracellular amines, resulting in stable, well-retained fluorescent staining that can be fixed with aldehyde fixatives. Excess unconjugated reagent passively diffuses to the extracellular medium, where it can be quenched with complete media and washed away.

The approximate excitation and emission peaks of Cell-ID™ Violet reagent after hydrolysis are 405 nm and 450 nm, respectively. Cells labeled with Cell-ID™ Violet reagent can be visualized by fluorescence microscopy using standard DAPI filter sets or analyzed by flow cytometry in an instrument equipped with a 405 nm excitation source.

规格: 1. Platform: Flow Cytometer
2. Detection Method: Fluorescent
3. Ex/Em: 405/450 nm
应用: Cell generation analysis by flow cytometry.
组分: 1. Component A: 10 vials Violet
2. Component B: 500 µl anhydrous DMSO
储存条件:
At -20°C and Protect from light
文档:
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成功案例

Cell generation analysis with Violet Cell Proliferation Kit. Jurkat cells (~1×106 cells/ml) were stained with Violet dye (1 µM) on Day 0. The cells were cultured for 7 days. Fluorescence intensity was measured with FACS flow cytometer.

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