LiScript™ First-Strand cDNA Synthesis Kit (+gDNA wiper) (50 rxns)

• LiScript™ Reverse Transcriptase makes reverse transcription reaction more efficient, with more full-length cDNA can be obtained.

• Reaction products are applicable to subsequent PCR, qPCR and PCR cloning.

• 1 pg-2.5 μg total RNA can be detected.

• The kit includes the novel genetically engineered Complete Reverse Transcriptase in a convenient master mix solution. Therefore operation is simple, and the risk of contamination is minimized.

LiScript™ Reverse Transcriptase is a sequence-mutated reverse transcriptase based upon the structure of M-MLV (RNase H-) Reverse Transcriptase. LiScript™ Reverse Transcriptase substantially increases its thermal stability: its half-life exceeds more than 240 minutes at 50°C, and the activity remains stable for a much longer time at 55°C, which makes it highly suitable for RNA template with complex secondary structure. Furthermore, LiScript™ increases more point mutations, to further elevated the affinity of the template and progression, makes reverse transcription reaction more efficient, with more full-length cDNA can be obtained and as long as 20 kb cDNA can be synthesized, and for the common reverse transcriptase inhibitor has higher tolerance, reverse transcription reaction is very suitable for plant tissue RNA.

LiScript™ First-Strand cDNA Synthesis Kit (+gDNA wiper) contains all components needed to synthesize high-quality 1st strand cDNA. Reaction products are applicable to subsequent PCR, qPCR and PCR cloning. The pre-treatement of 4× gDNA wiper Mix ensures RNA template completely free of residual genomic DNA, which ensures quantitative results more reliable. 2× RT Mix contains optimized buffer system and dNTP; Enzyme Mix contains LiScript™ Reverse Transcriptase and RNase inhibitor. Oligo (dT)18 and Random hexamers can be chosen as primers for reverse transcription as needed. Compare with Oligo dT18, Oligo dT23VN in Kit has stronger anchoring ability for Poly A+ RNA, which makes the reverse transcription efficiency is higher. Choose Oligo dT23VN accordingly, Random hexamers or gene specific primers for reverse transcription primer.

1. RNase free ddH2O:  1 ml
2. 4 × gDNA wiper Mix: 200 μl
3. 10× RT Mix:  120 μl
4. LiScript™ Enzyme Mix:  100 μl
5. Oligo dT23 (50 μM):  50 μl
6. Random hexamers (50 ng/ μl):  50 μl