IntactProtein™ 细胞组织裂解试剂盒 (20 ml)

成功案例

Figure1. 与RIPA缓冲液相比，IntactProtein™裂解试剂盒在提取大尺寸蛋白质方面表现出优异的性能

A. 分别使用IntactProtein™裂解试剂盒和RIPA缓冲液裂解HeLa细胞，并使用mTOR（289 kDa）抗体进行蛋白免疫印迹，裂解物上样量为50μg。
B. HT1080细胞裂解物（50 μg）在如图所示处理的冰上裂解，并用抗SMRT（160，270 kDa）抗体印迹。注意，超声将SMRT片段化为更小的肽。

Figure2. IntactProtein™ 裂解试剂盒在从组织和细胞中提取蛋白质方面表现同样出色

A. 使用 IntactProtein™ 裂解试剂盒裂解 HeLa 细胞和小鼠大脑（一式两份）。使用mTOR (289 kDa)抗体对50 pg总裂解物进行免疫印迹。
B. 用抗GAPDH (37 kDa)抗体对(A)中的相同裂解物进行印迹。 请注意，IntactProtein™ 裂解试剂盒适用于从细胞和组织中提取大分子量和小分子量的蛋白质。

Figure3. IntactProtein™ 裂解试剂盒能很好地保护蛋白质翻译后修饰 (PTM)。

A-B. 使用 IntactProtein™裂解试剂盒对提取Hela细胞，Hela细胞无血清培养16小时后用胰岛素(150 nM) 处理 5 分钟，随后使用IntactProtein™ 裂解试剂盒提取蛋白。 分别使用p-MTOR (Ser2448) (A) 和 mTOR (B) 抗体对50 μg 完全产物进行蛋白免疫印迹。
C. 分别使用 RIPA 缓冲液或 IntactProtein™ 裂解试剂盒裂解 HeLa 细胞。 使用抗 O-连接 B-N-乙酰葡糖胺 (O-GcNAc)（糖基化）抗体对细胞裂解物进行印迹。

发表文章

1. PPARγ Acetylation inAdipocytes Exacerbates BAT Whitening and Worsens Age-AssociatedMetabolic Dysfunction. 
发表期刊：Cell        产品：IntactProtein™ Cell-Tissue Lysis Kit

2. Salidroside reducesneuropathology in Alzheimer's disease models by targeting NRF2/SIRT3pathway. 
发表期刊：Cell Biosci         产品：IntactProtein™ Cell-Tissue Lysis Kit

3. Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity.  
发表期刊：Advanced Science        产品：IntactProtein™ Cell-Tissue Lysis Kit

4. O-GlcNAcylation is a gatekeeper of porcine myogenesis.  
发表期刊：J Anim Sci        产品：IntactProtein™ Cell-Tissue Lysis Kit

5. PPARγAcetylation Orchestrates Adipose Plasticity and Metabolic Rhythms. 
发表期刊：AdvSci (Weinh)        产品：IntactProtein™ Cell-Tissue Lysis Kit

客户评价

"I was always having trouble dealing with extraction of large-sized proteins such as mTOR (MW 289) from cultured cells. My problem was that without sonication, the extraction efficiency of large proteins was low; with sonication, the large-sized proteins were fragmented into smaller-sized peptides. This dilemma was finally solved by using IntactProtein™ Cell-Tissue Lysis Kit. With this kit, I obtained excellent results without losing phosphorylation signals. Moreover, this kit was easy to use by simply mixing reagent A and B and lysing the cells for 15 min on ice."

"We have tested your IntactProtein™ Cell-Tissue Lysis Kit in liver, adipose tissue, and cultured cells. It worked really well! Extracting proteins from fat is always a headache. Your buffer gave us high protein yields and sharp protein bands. We’d like to switch to your buffer"