产品优势: | • 高特异性:高封闭Taq抗体和优化的PCR缓冲液双重作用大大降低了引物二聚体的产生。 • 室温稳定:在储存和运输过程中,性能不容易随温度变化而降低;完全室温配制体系。 • 高GC扩增:优化的缓冲液配方可高效扩增GC 含量高达 80% 的模板,从而简化引物设计。 • 宽线性范围:可在8个模板梯度线性范围内展示很高的重复性。 |
描述: | LiQuant™ Ultra Green qPCR Master Mix一款经典定量 PCR 试剂盒,基于 SYBR Green I 染料法的 2×预混液,包含除引物和模板外的所有组分。本产品采用抗体法热启动的 Taq 酶,可进行高特异、高稳定的定量 PCR 分析。该酶通过特殊配方优化,适用于高 GC 含量模板的扩增,可在 37℃ 下 7 天性能保持不变,具有很高的稳定性,推荐室温加样操作。该试剂中已预混 ROX,可兼容大部分定量 PCR 仪。 |
组分: | 1. LiQuant™ Ultra Green qPCR Master Mix: 20 ml |
存储条件: | 2-8℃保存1年 |
文档: | 说明书 MSDS |
Figure 1. Comparison of the specificity between LiQuant™ Ultra and Brand N
Template DNA: Eight 10×Dilutes of the pET28a plasmid with Bacillus badius phenylalanine dehydrogenase gene.
Primer: Forward primer AGGAAGCCGATGTGTTCGTT Reverse primer TTCCGCTTGCTGGTACACTT
From the melting curve, it shows that LiQuant™ Ultra exhibits a single peak under both low and high concentration templates, while under low concentration templates, Brand N exhibits non-specific amplification.
Figure 2. High stability verification
Template DNA: Eight 10×Dilutes of the pET28a plasmid with Bacillus badius phenylalanine dehydrogenase gene.
Primer: Forward primer AGGAAGCCGATGTGTTCGTT Reverse primer TTCCGCTTGCTGGTACACTT
From the amplification curve, it shows that the LiQuant™ stored at 37 ℃ and at -20 ℃ have the same curve, and the Cq value is basically similar. From the standard curve, it shows that the PCR efficiency of LiQuant™ at different stored temperatures are both at 95% -100%, and the R2 value is 0.999.