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| 产品优势： | • Simple: Labeling is complete in two steps • Efficient: No denaturation steps or harsh treatment required • Content-rich results: Better preservation of cell morphology, antigen structure, and DNA integrity • Consistent: Not dependent on variable antibody lots for detection |
| 描述： | LiFluor™ 594 EdU Imaging Kit is a superior alternative to the traditional BrdU cell proliferation assay that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable LiFluor™ dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol. |
| 规格： |
1. Platform: Fluorescence Microscope 2. Detection Method: Fluorescent 3. Ex/Em: 590/615 nm |
| 应用： | Cell proliferation analysis in vitro and in vivo. |
| 组分： |
1. Component A: EdU (2×1 ml) 2. Component B: LiFluor 555 azide (100 µl) 3. Component C: EdU reaction buffer (50 ml) 4. Component D: CuSO4 (1 ml) 5. Component E: EdU buffer additive (200 mg) 6. Component F: Hoechst 33342 (70 µl) |
| 储存条件： |
At -20°C and Protect from light |
| 文档： |
请联系客服索取产品说明书 |
成功案例
Detection of cell proliferation in cell. A549 cells were treated with 10 µM EdU for 2 hr, then detected with LiFluor™ 594 azide (red), cells were counterstained with DAPI (blue).
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